Open Access

Genome analysis of Desulfotomaculum kuznetsovii strain 17T reveals a physiological similarity with Pelotomaculum thermopropionicum strain SIT.

  • Michael Visser
  • , Petra Worm
  • , Gerard Muyzer
  • , Inês A.C. Pereira
  • , Peter J. Schaap
  • , Caroline M. Plugge
  • , Jan Kuever
  • , Sofiya N. Parshina
  • , Tamara N. Nazina
  • , Anna E. Ivanova
  • , Rizlan Bernier-Latmani
  • , Lynne A. Goodwin,
  • , Nikos C. Kyrpides
  • , Tanja Woyke
  • , Patrick Chain,
  • , Karen W. Davenport,
  • , Stefan Spring
  • , Hans-Peter Klenk
  • and Alfons J.M. Stams,

DOI: 10.4056/sigs.3627141

Received: 15 April 2013

Published: 15 April 2013

Abstract

Desulfotomaculum kuznetsovii is a moderately thermophilic member of the polyphyletic spore-forming genus Desulfotomaculum in the family Peptococcaceae. This species is of interest because it originates from deep subsurface thermal mineral water at a depth of about 3,000 m. D. kuznetsovii is a rather versatile bacterium as it can grow with a large variety of organic substrates, including short-chain and long-chain fatty acids, which are degraded completely to carbon dioxide coupled to the reduction of sulfate. It can grow methylotrophically with methanol and sulfate and autotrophically with H2 + CO2 and sulfate. For growth it does not require any vitamins. Here, we describe the features of D. kuznetsovii together with the genome sequence and annotation. The chromosome has 3,601,386 bp organized in one contig. A total of 3,567 candidate protein-encoding genes and 58 RNA genes were identified. Genes of the acetyl-CoA pathway, possibly involved in heterotrophic growth with acetate and methanol, and in CO2 fixation during autotrophic growth are present. Genomic comparison revealed that D. kuznetsovii shows a high similarity with Pelotomaculum thermopropionicum. Genes involved in propionate metabolism of these two strains show a strong similarity. However, main differences are found in genes involved in the electron acceptor metabolism.

Keywords:

Thermophilic spore-forming anaerobessulfate reductionautotrophicmethylotrophicPeptococcaceaeClostridiales

Introduction

Desulfotomaculum kuznetsovii strain 17T (VKM B-1805; DSM 6115) is a moderately thermophilic sulfate-reducing bacterium isolated from deep subsurface thermal mineral water [1]. It grows with a wide range of substrates, including organic acids, such as long-chain fatty acids, short-chain fatty acids (butyrate, propionate, acetate), lactate, pyruvate, fumarate and succinate as well as ethanol and methanol. These substrates are degraded to CO2 coupled to sulfate reduction. The strain is also able to grow autotrophically with H2/CO2 and sulfate and to ferment pyruvate and fumarate. For growth, D. kuznetsovii has no vitamin requirement.

Desulfotomaculum is a genus of Gram-positive, spore-forming anaerobes that is phylogenetically and physiologically very diverse. The genus is poorly studied physiologically, while its members are known to play an important role in the carbon and sulfur cycle in a variety of often adverse environments. The genus is divided phylogenetically into different sub-groups [2,3]. To get a thorough understanding of the evolutionary relationship of the different Desulfotomaculum sub-groups and the physiology of the individual species, it is important to have genome sequence information. Here, we present a summary of the features of D. kuznetsovii strain 17T, together with the description of the complete genomic sequencing and annotation. Moreover, we describe a physiological and genomic comparison of D. kuznetsovii strain 17T and Pelotomaculum thermopropionicum strain SIT, because phylogenetically P. thermopropionicum is the closest related organism with validly published name that has a completely sequenced genome. However, the two strains have different physiological traits. For example, P. thermopropionicum is not able to grow by sulfate reduction, but is able to grow in syntrophy with methanogens. D. kuznetsovii lacks this ability. By comparing the genomes of the two bacteria we were able to identify the main similarities and differences.

Classification and features

D. kuznetsovii is a member of the phylum Firmicutes. Phylogenetic analysis of the 16S rRNA genes of D. kuznetsovii shows that it clusters in Desulfotomaculum cluster 1. This cluster not only contains Desulfotomaculum species, but also members of the genera Sporotomaculum, Cryptanaerobacter and Pelotomaculum. D. kuznetsovii is part of sub-group 1c together with D. solfataricum, D. luciae, D. thermosubterraneum, D. salinum, D. australicum, and D. thermocisternum, while Pelotomaculum species belong to sub-group 1h (Figure 1) [2].

Figure 1

Neighbor joining tree based on 16S rRNA sequences showing the phylogenetic affiliation of Desulfotomaculum and related species divided in the subgroups of Desulfotomaculum cluster 1. D. kuznetsovii is printed in bold type. The sequences of different Thermotogales were used as outgroup, but were pruned from the tree. Closed circles represent bootstrap values between 75 and 100%. The scale bar represents 10% sequences difference.

D. kuznetsovii cells are rod-shaped (1.0-1.4 x 3.5-5 μm) with rounded ends and peritrichous flagella [Figure 2]. Spores of D. kuznetsovii are spherical (1.3 μm in diameter) and centrally located causing swelling of the cells. D. kuznetsovii grows between 50 and 85°C, but the optimal growth temperature is 60-65°C. The substrates D. kuznetsovii can grow with are completely oxidized to CO2. Suitable electron acceptors are sulfate, thiosulfate and sulfite. D. kuznetsovii is also able to grow by fermentation of pyruvate and fumarate. A summary of the classification and general features of D. kuznetsovii is presented in Table 1 [1].

Figure 2

Scanning electron microscopic photograph of D. kuznetsovii.

Table 1

Classification and general features of D. kuznetsovii DSM 6115 according to the MIGS recommendations [4].

MIGS ID

   Property

  Term

   Evidence codea

   Current classification

  Domain Bacteria

   TAS [5]

  Phylum Firmicutes

   TAS [6-8]

  Class Clostridia

   TAS [9,10]

  Order Clostridiales

   TAS [11,12]

  Family Peptococcaceae

   TAS [11,13]

  Genus Desulfotomaculum

   TAS [11,14,15]

  Species Desulfotomaculum kuznetsovii

   TAS [1,16]

  Type strain 17

   Gram stain

  Positive

   TAS [1]

   Cell shape

  Rods

   TAS [1]

   Motility

  peritrichous flagella

   TAS [1]

   Sporulation

  oval, terminal or subterminal, slightly swelling the cell.

   TAS [1]

   Temperature range

  50-85°C

   TAS [1]

   Optimum temperature

  60-65°C

   TAS [1]

   Carbon source

  CO2 (autotrophic) and organic substrates (heterotrophic)

   TAS [1]

   Energy source

  Sulfate-dependent growth and fermentative growth with pyruvate and fumarate.

   TAS [1]

   Electron acceptor

  Sulfate, thiosulfate and sulfite.

   TAS [1]

MIGS-6

   Habitat

  Geothermal groundwater, sediment and hot solfataric fields.

   TAS [1,17,18]

MIGS-6.3

   Salinity

  2-3% NaCl

   TAS [1]

MIGS-22

   Oxygen

  Obligate anaerobes

   TAS [1]

MIGS-15

   Biotic relationship

  Free living

   TAS [1]

MIGS-14

   Pathogenicity

  None

MIGS-4

   Geographic location

  Sukhumi, Georgia

   TAS [1]

MIGS-5

   Sample collection time

  1987 or before

   TAS [1]

MIGS-4.1

   Latitude

  43.009

   TAS [1]

MIGS-4.2

   Longitude

  40.989

   TAS [1]

MIGS-4.3

   Depth

  2800-3250 m

   TAS [1]

Evidence codes - TAS: Traceable Author Statement (i.e., a direct report exists in the literature); NAS: Non-traceable Author Statement (i.e., not directly observed for the living, isolated sample, but based on a generally accepted property for the species, or anecdotal evidence). Evidence codes are from the Gene Ontology project [19].

Genome sequencing and annotation

Genome project history

D. kuznetsovii was selected for sequencing in the DOE Joint Genome Institute Community Sequencing Program 2009, proposal 300132_795700 'Exploring the genetic and physiological diversity of Desulfotomaculum species', because of its phylogenetic position in one of the Desulfotomaculum sub-groups, its important role in bioremediation, and its ability to use propionate, acetate and methanol for growth. The genome project is listed in the Genome OnLine Database (GOLD) [20] as project Gc01781, and the complete genome sequence was deposited in Genbank. Sequencing, finishing and annotation of the D. kuznetsovii genome were performed by the DOE Joint Genome Institute (JGI). A summary of the project information is shown in Table 2.

Table 2

Genome sequencing project information

MIGS ID

   Property

   Term

MIGS-31

   Finishing quality

   Finished

MIGS-28

   Libraries used

   Four genomic libraries: one Illumina shotgun library,   one 454 standard library, two paired end 454 libraries

MIGS-29

   Sequencing platforms

   Illumina GAii, 454 Titanium

MIGS-31.2

   Fold coverage

   158.2 × illumina; 30.6 × pyrosequencing

MIGS-30

   Assemblers

   VELVET version 0.7.63; Newbler version 2.3;    phrap version SPS - 4.24

MIGS-32

   Gene calling method

   Prodigal 1.4, GenePRIMP

   INSDC ID

   CP002770.1

   Genome Database release

   July 20, 2012

   Genbank Date of Release

   May 24, 2011

MIGS-13

   GOLD ID   NCBI project ID   Source material identifier

   Gc01781   48313   DSM 6115T

   Project relevance

   Obtain insight into the phylogenetic and physiological    diversity of Desulfotomacum species, and bioremediation.

Growth conditions and DNA isolation

D. kuznetsovii was grown anaerobically at 60oC in bicarbonate buffered medium with propionate and sulfate as substrates [1]. DNA of cell pellets was isolated using the standard DOE-JGI CTAB method recommended by the DOE Joint Genome Institute (JGI, Walnut Creek, CA, USA). In short, cells were resuspended in TE (10 mM tris; 1 mM EDTA, pH 8.0). Subsequently, cells were lysed using lysozyme and proteinase K, and DNA was extracted and purified using CTAB and phenol:chloroform:isoamylalcohol extractions. After precipitation in 2-propanol and washing in 70% ethanol, the DNA was resuspended in TE containing RNase. Following a quality and quantity check using agarose gel electrophoresis in the presence of ethidium bromide, and spectrophotometric measurement using a NanoDrop ND-1000 spectrophotometer (NanoDrop® Technologies, Wilmington, DE, USA).

Genome sequencing and assembly

The genome was sequenced using a combination of Illumina and 454 sequencing platforms. All general aspects of library construction and sequencing can be found at the JGI website [21]. Pyrosequencing reads were assembled using the Newbler assembler (Roche). The initial Newbler assembly consisting of 81 contigs in five scaffolds was converted into a phrap [22] assembly by making fake reads from the consensus, to collect the read pairs in the 454 paired end library. Illumina GAii sequencing data (570.2 Mb) was assembled with Velvet [23] and the consensus sequences were shredded into 1.5 kb overlapped fake reads and assembled together with the 454 data. The 454 draft assembly was based on 134.6 Mb 454 draft data and all of the 454 paired end data. Newbler parameters are -consed -a 50 -l 350 -g -m -ml 20. The Phred/Phrap/Consed software package [22] was used for sequence assembly and quality assessment in the subsequent finishing process. After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected with gapResolution [21], Dupfinisher [24], or sequencing cloned bridging PCR fragments with subcloning. Gaps between contigs were closed by editing in Consed, by PCR and by Bubble PCR primer walks (J.-F. Chang, unpublished). A total of 400 additional reactions and one shatter library were necessary to close gaps and to raise the quality of the finished sequence. Illumina reads were also used to correct potential base errors and increase consensus quality using a software Polisher developed at JGI [25]. The error rate of the completed genome sequence is less than 1 in 100,000. Together, the combination of the Illumina and 454 sequencing platforms provided 188.8 × coverage of the genome. The final assembly contained 323,815 pyrosequence and 15,594,144 Illumina reads.

Genome annotation

Genes were identified using Prodigal [26] as part of the Oak Ridge National Laboratory genome annotation pipeline, followed by a round of manual curation using the JGI GenePRIMP pipeline [27]. The predicted CDSs were translated and used to search the National Center for Biotechnology Information (NCBI) nonredundant database, UniProt, TIGR-Fam, Pfam, PRIAM, KEGG, COG, and InterPro databases. Additional gene prediction analysis and functional annotation was performed within the Integrated Microbial Genomes - Expert Review (IMG-ER) platform [28].

Genome properties and genome comparison with other strains

The genome of D. kuznetsovii consists of a circular chromosome of 3,601,386 bp with 54.88% GC content (Table 3 and Figure 3). Pseudogenes comprise 4.66% of the genes identified. Of the 3,625 genes predicted, 3,567 are protein-coding genes of which 2,560 are assigned to COG functional categories. The distribution of these genes into COG functional categories is presented in Table 4.

Table 3

Genome statistics

Attribute

   Value

   % of totala

Genome size (bp)

   3,601,386

   100.00

Genome coding region (bp)

   3,057,959

   84.91

Genome G+C content (bp)

   1,976,601

   54.88

Total genes

   3,625

   100.00

RNA genes

   58

   1.60

Protein-coding genes

   3,567

   98.40

Genes in paralog clusters

   1,373

   37.88

Genes assigned to COGs

   2,560

   70.62

Pseudo genes

   169

   4.66

Genes with signal peptides

   582

   16.06

Genes with transmembrane helices

   748

   20.63

Figure 3

Graphical map of the chromosome of D. kuznetsovii. From outside to the center: Genes on the forward strand (colored by COG categories), Genes on the reverse strand (colored by COG categories), RNA genes (tRNAs green, rRNAs red, other RNAs black), GC content, GC skew.

Table 4

Number of genes associated with the general COG functional categories

Code

   Value

   %agea

   Description

J

   148

   5.32

   Translation

A

   0

   0.00

   RNA processing and modification

K

   184

   6.61

   Transcription

L

   207

   7.44

   Replication, recombination and repair

B

   2

   0.07

   Chromatin structure and dynamics

D

   60

   2.16

   Cell cycle control, mitosis and meiosis

Y

   0

   0.00

   Nuclear structure

V

   35

   1.26

   Defense mechanisms

T

   177

   6.36

   Signal transduction mechanisms

M

   122

   4.38

   Cell wall/membrane biogenesis

N

   79

   2.84

   Cell motility

Z

   2

   0.07

   Cytoskeleton

W

   0

   0.00

   Extracellular structures

U

   75

   2.69

   Intracellular trafficking and secretion

O

   81

   2.91

   Posttranslational modification, protein turnover, chaperones

C

   261

   9.38

   Energy production and conversion

G

   106

   3.81

   Carbohydrate transport and metabolism

E

   197

   7.08

   Amino acid transport and metabolism

F

   55

   1.98

   Nucleotide transport and metabolism

H

   158

   5.68

   Coenzyme transport and metabolism

I

   89

   3.20

   Lipid transport and metabolism

P

   127

   4.56

   Inorganic ion transport and metabolism

Q

   122

   3.58

   Secondary metabolites biosynthesis, transport and catabolism

R

   331

   11.89

   General function prediction only

S

   257

   9.23

   Function unknown

-

   1,065

   29.38

   Not in COGs

The genome of D. kuznetsovii has 58 RNA genes of which, three are 16S rRNA genes. This is one more than the previously described rrnA and rrnB [29]. These two rRNA genes contained two large inserts. One at the variable 5’terminal region and one at the variable 3’terminal region. The main differences between the two rRNA genes were found in these inserts. These inserts were hypothesized to be involved in the operation of ribosomes at high temperatures. However, more research is needed to assess the function of these inserts. All three rRNA genes of D. kuznetsovii have a size of approximately 1,700 nucleotides. This suggests that the third rRNA gene might also contain inserts. Alignment of the 16S rRNA genes confirmed the presence of inserts in all three 16S rRNA genes (data not shown).

BLAST analysis [30,31] of the genes of D. kuznetsovii against genes in the KEGG Sequence Similarity DataBase revealed similarity with other Desulfotomaculum strains (Table 5), D. acetoxidans, D. carboxydivorans, “D. reducens” and D. ruminis, but interestingly also with non-Desulfotomaculum strains. D. kuznetsovii contains 873 genes with high similarity to genes of Pelotomaculum thermopropionicum, which is more than to any of the sequenced Desulfotomaculum species. Moreover, we identified the conserved proteins of D. kuznetsovii across three related fully sequenced species (Table 6). The bidirectional best blast hits showed that despite the smaller genome of P. thermopropionicum it contained more homologous predicted proteins with D. kuznetsovii (1,406) compared to D. acetoxidans (1,309) and “D. reducens” (1330). This suggests a strong physiological similarity between D. kuznetsovii and P. thermopropionicum.

Table 5

Taxonomic distribution of the top KEGG hits of D. kuznetsovii genes based on BLAST against KEGG database.

Kingdom

   Category

   Species

  Hits

Archaea

  91

   Crenarchaeota

  9

   Euryarchaeota

  81

   Thaumarchaeota

  1

Bacteria

  2,963

   Acidobacteria

  2

   Actinobacteria

  16

   Alphaproteobacteria

  13

   Bacteroidetes

  5

   Betaproteobacteria

  14

   Cyanobacteria

  19

   Deinococcus-Thermus

  16

   Deltaproteobacteria

  62

   Epsilonproteobacteria

  1

   Firmicutes

  2,728

   Ammonifex degensii

  170

   Carboxydothermus hydrogenoformans

  58

   Desulfotomaculum acetoxidans

  310

   Candidatus Desulforudis audaxviator

  154

   Desulfotomaculum carboxydivorans

  268

   Desulfotomaculum reducens

  111

   Desulfotomaculum ruminis

  132

   Moorella thermoacetica

  183

   Pelotomaculum thermopropionicum

  873

   Thermincola potens JR

  104

   Fusobacteria

  2

   Gammaproteobacteria

  12

   Green nonsulfur bacteria

  20

   Green sulfur bacteria

  4

   Hyperthermophilic bacteria

  31

   OtherProteobacteria

  1

   Spirochaetes

  9

   Synergistetes

  6

   Verrucomicrobia

  2

Eukaryotes

  3

   Plants

  1

   Protists

  2

Null

  342

Total

  3,399

Species that had more than 50 genes similar to D. kuznetsovii were included in this table, others were only summarized in categories.

Table 6

Proteins of D. kuznetsovii conserved across three related species with fully sequenced genomes.

Subject DB Input Query

   D. acetoxidans

   D. kuznetsovii

   D. reducens

    P. thermopropionicum

Desulfotomaculum acetoxidans

   4,068

   1,539

   1,525

    1,486

   1,309

   1,316

    1,255

Desulfotomaculum kuznetsovii

   1,509

   3,398

   1,518

    1,645

   1,309

   1,330

    1,406

Desulfotomaculum reducens

   1,537

   1,571

   3,276

    1,438

   1,316

   1,330

    1,211

Pelotomaculum thermopropionicum

   1,430

   1,600

   1,395

    2,919

   1,255

   1,406

   1,211

BLAST analyses were performed using standard settings and best hits were filtered for 40% identity over an alignment length of 75 amino acids as a minimum requirement. The values show the number of predicted proteins that are homologous to the query species in each row. The number of similar proteins obtained with a unidirectional BLAST is indicated in light blue. Bidirectional best blast hits are indicated in dark blue. Proteomes were obtained from Web Site. Accession numbers are in parenthesis: D. acetoxidans (NC_013216); D. kuznetsovii (NC_015573); “D. reducens” (NC_009253); Pelotomaculum thermopropionicum (NC_009454).

Insights into the genome

Involvement of the acetyl-coA pathway in growth with acetate and methanol

D. kuznetsovii oxidizes acetate completely to CO2. The pathway of acetate degradation has not been studied yet, but sulfate reducers may employ the tricarboxylic acid (TCA) cycle or the acetyl-CoA pathway for acetate degradation, as exemplified by Desulfobacter postgatei and Desulfobacca acetoxidans, respectively [32]. Most genes predicted to code for enzymes of the TCA cycle are present in the genome of D. kuznetsovii, but genes with similarity to those coding for an ATP-dependent citrate synthase and isocitrate dehydrogenase are missing. This suggests that the TCA cycle is not complete and that the TCA cycle enzymes have mainly an anabolic function or a function in other catabolic pathways, such as the propionate degradation pathway. Genes with similarity to those coding for enzymes involved in the acetyl-CoA pathway are all present in the genome of D. kuznetsovii (Figure 4), which suggests its involvement in acetate oxidation. However, there are no genes similar to those that code for acetate kinase and phosphate acetyltransferase present in the genome. The reaction from acetate to acetyl-CoA is likely performed by acetyl-CoA synthetase (Desku_1241).

Figure 4

Pathway of acetate oxidation to CO2 by D. kuznetsovii. Enzymes in this figure are in bold italic and their locus tags are included. Genes with the locus tags Desku_1488 and Desku_1490 putatively code for the small subunit and the large subunit of the iron-sulfur protein, respectively. This protein is involved in transferring the methyl from acetyl-CoA to tetrahydrofolate. Abbreviations: A-CoA S, acetyl-CoA synthetase; AcsA, carbon-monoxide dehydrogenase; AcsB, acetyl-CoA synthase; CFeSP, iron-sulfur protein; CH3, methyl; THF, tetrahydrofolate; MeTr, methyltransferase.

D. acetoxidans is an acetate-oxidizing Desulfotomaculum species, positioned in sub-group 1e (Figure 1), that also uses the acetyl-CoA pathway for acetate oxidation to CO2 [33]. The genes involved in acetate oxidation in D. acetoxidans are similar to those in D. kuznetsovii, but there are some exceptions. The genome of D. acetoxidans does not contain a gene that putatively codes for acetyl-CoA synthetase, similar to D. kuznetsovii, but contains genes that putatively code for an acetate kinase and a phosphate acetyltransferase [34]. Additionally, putative carbon-monoxide dehydrogenase complex coding genes involved in the acetyl-CoA pathway show differences between the two Desulfotomaculum species. D. kuznetsovii lacks a ferredoxin coding gene that is located between cooC (Desku_1493) and acsE (Desku_1487), which in contrast is present in the genome of D. acetoxidans (Dtox_1273). Moreover, three genes similar to heterodisulfide reductase encoding genes (Desku_1486-1484) are located upstream of acsE in D. kuznetsovii, which is not the case in the genome of D. acetoxidans.

Methanol metabolism

Growth of D. kuznetsovii with methanol and sulfate was studied [35]. In that study the activity of methyltransferase, an enzyme that is involved in methanol metabolism in methanogens and acetogens [36,37], could not be assessed, while low activities of an alcohol dehydrogenase could be measured. An alcohol dehydrogenase with a molecular mass of 42 kDa was partially purified and showed activity with methanol [35]. The genome of D. kuznetsovii contains several alcohol dehydrogenase genes (Desku_0165, 0619, 0624, 0628, 2955, 3082) that each code for an enzyme with a size of approximately 42 kDa. In the genome, genes with similarity to those coding for a methanol methyltransferase mtaA (Desku_0050, 0055, 0060), mtaB (Desku_0051) and mtaC (Desku_0048, 0049, 0052, 0056) were also found, suggesting a methanol metabolism as described in Moorella thermoacetica [36]. Further studies are needed to obtain information about the diversity of the methanol-degradation pathways in D. kuznetsovii.

Comparison of D. kuznetsovii and P. thermopropionicum genomes

Genomic comparison revealed that a large number of D. kuznetsovii genes show similarity to genes of Pelotomaculum thermopropionicum, a syntrophic propionate-oxidizing thermophile (Table 5 and 6). Interestingly, among them are genes that putatively code for enzymes involved in propionate metabolism (Table 7). Moreover, the genetic organization of the methylmalonyl-CoA (mmc) cluster in the genome of both bacteria is similar (Figure 5). However, D. kuznetsovii lacks tps, mmcA and mmcM in the mmc cluster. mmcA codes for a response regulator and mmcM for pyruvate ferredoxin oxidoreductase.

Table 7

Genes in D. kuznetsovii that are annotated as enzymes involved in propionate metabolism.

   Homologous protein in   P. thermopropionicum

Gene symbol

  Locus tag

   Function

  Identity (%)

   Locus tag

sdhB

  Desku_0434

   Succinate dehydrogenase, FeS protein

  76

   PTH_1018

sdhA

  Desku_0435

   Succinate dehydrogenase, flavoprotein

  76

   PTH_1017

sdhC

  Desku_0436

   Succinate dehydrogenase, cytochrome b

  51

   PTH_1016

citE

  Desku_1348

   Citrate lyase

  57

   PTH_1335

sdhA

  Desku_1353

   Succinate dehydrogenase, flavoprotein

  83

   PTH_1491

sdhB

  Desku_1354

   Succinate dehydrogenase, FeS protein

  75

   PTH_1490

mmcB

  Desku_1358

   Fumarase, N-terminal domain

  73

   PTH_1356

mmcC

  Desku_1359

   Fumarase, C-terminal domain

  77

   PTH_1357

mmcD2

  Desku_1361

   Succinyl-CoA synthetase, alpha subunit

  78

   PTH_1359

mmcE

  Desku_1362

   Methylmalonyl-CoA mutase,   N-terminal domain

  77

   PTH_1361

mmcF

  Desku_1363

   Methylmalonyl-CoA mutase,   C-terminal domain

  82

   PTH_1362

mmcG

  Desku_1364

   Methylmalonyl-CoA epimerase

  86

   PTH_1363

mmcH

  Desku_1365

   Methylmalonyl-CoA decarboxylase,   alpha subunit

  75

   PTH_1364

mmcI

  Desku_1366

   Methylmalonyl-CoA decarboxylase,   epsilon subunit

  82

   PTH_1365

mmcJ

  Desku_1367

   Methylmalonyl-CoA decarboxylase,   gamma subunit

  56

   PTH_1366

mmcK

  Desku_1368

   Malate dehydrogenase

  75

   PTH_1367

mmcL

  Desku_1369

   Transcarboxylase 5S subunit

  66

   PTH_1368

pykF

  Desku_1651

   Pyruvate kinase

  73

   PTH_2214

ppsA

  Desku_2615

   Pyruvate phosphate dikinase

  78

   PTH_0903

citE

  Desku_2747

   Citrate lyase

  56

   PTH_1335

Corresponding homologs in P. thermopropionicum are included.

Figure 5

Gene organization of the mmc cluster in D. kuznetsovii and P. thermopropionicum. Names of the genes can be found in table 6, except for tps, which is a transposase gene.

Based on 16S rRNA gene sequences, D. kuznetsovii and P. thermopropionicum group in cluster group c and h of the Desulfotomaculum cluster 1, respectively (see Figure 1). P. thermopropionicum is known for its ability to grow with propionate and ethanol in syntrophic association with methanogens. It is not able to grow by sulfate respiration, despite the presence of sulfate reduction genes in the genome [38]. In contrast, D. kuznetsovii is able to grow with propionate (Figure 6) and ethanol with sulfate. However, in the absence of sulfate, it cannot grow in syntrophic association with methanogens. Therefore, differences are expected in genes coding for hydrogenases, formate dehydrogenases, and those involved in sulfate reduction.

Figure 6

Propionate degradation pathway in D. kuznetsovii based on genomic data. Enzymes are depicted in bold italic. Next to these enzymes are the possible encoding genes, and their locus tags. GCT, Glutaconate CoA-transferase; MCD, Methylmalonyl-CoA decarboxylase; PCC, Propionyl-CoA carboxylase; MCE, Methylmalonyl-CoA epimerase; MCM, Methylmalonyl-CoA mutase; SCS, Succinyl-CoA synthetase; SDH, Succinate dehydrogenase; FHT, Fumarase; MDH, Malate dehydrogenase; OAD, Oxaloacetate decarboxylase; PFL, Pyruvate formate lyase.

Sulfate reduction genes: Figure 7 depicts the sulfate reduction pathway of the two strains. In the genome of D. kuznetsovii two genes (Desku_2103; Desku_3527) are annotated as phosphoadenosine phosphosulfate reductase encoding genes whose corresponding proteins might be involved in assimilatory sulfate metabolism. The P. thermopropionicum genome lacks these genes [39]. Instead, the P. thermopropionicum genome contains an adenylylsulfate kinase gene (PTH_0238). In the dissimilatory sulfate reduction pathway, the two strains both have genes that code for enzymes to reduce sulfate to H2S. However, P. thermopropionicum is missing the gene that codes for an adenylylsulfate reductase beta subunit, which is present in the D. kuznetsovii genome (Desku_1073). Moreover, the gene labeled as a dissimilatory sulfite reductase (dsr) alpha and beta subunit in the P. thermopropionicum genome (PTH_0242) is not similar to dsrA or dsrB from D. kuznetsovii or any other Desulfotomaculum strain. However, it has high similarity to the dsrC gene from D. kuznetsovii, indicating that it is not a dsrA or dsrB gene but a dsrC gene (data not shown). Therefore, the inability of P. thermopropionicum to grow by sulfate respiration is most likely caused by the absence of an adenylylsulfate reductase beta subunit encoding gene and the dsrAB genes.

Figure 7

Sulfate reduction pathway of D. kuznetsovii and P. thermopropionicum. Depicted in green are genes that code for sulfate reduction enzymes that are present in the genome. Dashed arrows indicate the presence of a subunit encoding gene, but not the presence of all genes required for the enzyme. Dashed dotted arrows are used when no genes were found for the reaction. Abbreviations: APS, adenylylsulfate; DP, diphosphate; PAPS, 3’-Phosphoadenylyl-sulfate (PAPS); redA, reduced acceptor; oxA, oxidized acceptor.

Hydrogenase and formate dehydrogenase genes: Schut and Adams (2009) [40] showed that the trimeric [FeFe]-hydrogenase from Thermotoga maritima oxidizes NADH and ferredoxin simultaneously to produce H2. Similar bifurcating / confurcating [FeFe]-hydrogenases and formate dehydrogenases are present in Syntrophobacter fumaroxidans and P. thermopropionicum [41]. Both generate NADH and ferredoxin during propionate degradation via the methylmalonyl-CoA pathway and might use confurcating hydrogenases and formate dehydrogenases to drive the unfavorable re-oxidation of NADH (E0’=-320mV) by the exergonic re-oxidation of ferredoxin (E0’=-398mV) to produce hydrogen (E0’= -414mV) or formate (E0’= -432mV) that are subsequently transferred to hydrogen and formate scavenging methanogens. Additionally, up-regulation of genes encoding hydrogenases and formate dehydrogenases in P. thermopropionicum was shown during syntrophic growth [42]. The P. thermopropionicum genome contains three [FeFe]-hydrogenases, one [NiFe]-hydrogenase and two formate dehydrogenases. One [FeFe]-hydrogenase (PTH_0668-0670) was shown to be down-regulated during syntrophic growth, while the other two [FeFe]-hydrogenases (PTH_1377-1379 and PTH_2010-2012) were up-regulated. The two formate dehydrogenases of P. thermopropionicum (I, PTH_1711-1714 and II, PTH_2645-2649) were both up-regulated during syntrophic growth [42]. According to TMHMM server v. 2.0 [43] formate dehydrogenase I of P. thermopropionicum has transmembrane helices. Therefore, it might play an essential role in the interspecies transfer of reducing equivalents in syntrophic growth.

The genome of D. kuznetsovii was screened for hydrogenase and formate dehydrogenase encoding gene clusters with BLAST analysis. Pfam search [44] was used to identify motifs in the amino acid sequences and the TMHMM Server v. 2.0 [43] was used to screen for transmembrane helices. The TatP 1.0 Server was used to screen for twin-arginine translocation (Tat) motifs in the N-terminus to predict protein localization in the cell [45]. The incorporation of selenocysteine (SeCys) was examined by RNA loop predictions with Mfold version 3.2 [46, 47]. The predicted RNA loop in the 50-100 bp region downstream of the UGA-codon was compared with the consensus loop described earlier [48].

Compared to P. thermopropionicum, D. kuznetsovii lacks membrane associated formate dehydrogenases and hydrogenases and also lacks [NiFe]-hydrogenase. This might explain why D. kuznetsovii cannot grow in syntrophic relation with methanogens. The genome of D. kuznetsovii indicates the presence of a confurcating selenocysteine-incorporated formate dehydrogenase (Desku_2987-2991), two trimeric confurcating [FeFe]-hydrogenases (Desku_2307-2309, Desku_2995-2997) and two [FeFe]-hydrogenases (Desku_0995, Desku_2934-2935) without NADH-binding sites (Figure 8). Several subunits of these enzymes are related to subunits of NADH dehydrogenase (complex I), including the NADH-binding proteins related to NuoF (Desku_2990, 2308 and 2996) and the electron transfer subunits related to NuoE (Desku_2991, 2935, and 2997) and to NuoG (Desku_2989). In three of the [FeFe]-hydrogenases this NuoG-like domain is fused with the catalytic subunit (Desku_2995, 2307 and 2934). Two of the multimeric hydrogenases are found next to [FeFe]-hydrogenases containing PAS-sensor domains (Desku_2932 and Desku_2994), suggesting they are involved in the regulation of the synthesis of those hydrogenases. All complexes are predicted to be cytoplasmic and not membrane bound.

Figure 8

Schematic representation of a putative confurcating formate dehydrogenase, two putative confurcating [FeFe]-hydrogenases and two ferredoxin re-oxidizing [FeFe]-hydrogenases in Desulfotomaculum kuznetsovii. Gene locus tag numbers and α-, β-, and γ-subunits are depicted. Moreover, predicted iron-sulfur clusters and metal-binding sites are indicated.

Apart from a possible involvement in the acetate oxidation pathway (Figure 4), it remains unclear for which purpose D. kuznetsovii uses its confurcating formate dehydrogenase and hydrogenases because our genome analysis indicates that pyruvate oxidation during propionate degradation generates formate instead of ferredoxin (Figure 6).

Vitamin synthesis

D. kuznetsovii is able to grow in medium without vitamins [1]. This indicates that D. kuznetsovii is able to synthesize all the vitamins that are required for its metabolism and that vitamin synthesis genes should be present in the genome. Vitamin B12 is essential for the methylmalonyl-CoA pathway and the acetyl-coA pathway. The biosynthesis of cobalamin (vitamin B12) is known to occur from uroporphyrinogen-III to adenosylcobalamin via two possible pathways, the aerobic and anaerobic pathway of the corrinoid ring [49,50]. The D. kuznetsovii genome contains all genes needed for the anaerobic pathway: cysGA (Desku_1520), cysGB (Desku_1460, Desku_1523), cbiA (Desku_1765, Desku_2368), cbiBCDEFGHJLPT (Desku_2369, 1459, 1468, 1467, 1464, 1463, 1462, 1461, 1465, 2370 and 1466, respectively), cobalt reductase (Desku_2757), btuR (Desku_0004, 1209), cobS (Desku_2367) and cobU (Desku_2371). Moreover, D. kuznetsovii has genes to convert glutamyl tRNA to uroporphyrinogen-III, hemABCDL (Desku_1522, 1518, 1521, 1520 and 1522, respectively). The genome also contains some unassigned cobalamin synthesis genes (P47K, Desku_0046, 0053; cbiM, Desku_2905), corrinoid transport proteins (Desku_0693, 702, 2237-2239, 2902-2904, 3025-3027) and, interestingly, two cobN genes (Desku_2189, 2227), genes involved in the aerobic pathway. It is unclear why D. kuznetsovii has these cobN genes, since all anaerobic pathway genes are present in the genome, and it is unclear if the products of these two genes are used for cobalamin synthesis by D. kuznetsovii.

Other vitamin synthesis genes present in the genome of D. kuznetsovii are genes involved in biotin synthesis (vitamin H) (Desku_1295-1297, 2246-2247, 2317), nicotinamide (vitamin B3) synthesis (Desku_0433, 0614, 0662, 0815, 1248, 1417, 1472, 1499, 1925, 1951, 3103, 3121, 3227, 3228, 3231, 3246, 3337), thiamin (vitamin B1) synthesis (0372, 0543, 0545, 2253, 2363, 2639), riboflavin (vitamin B2) synthesis (Desku_1244-1247), and pantothenate (vitamin B5) synthesis (Desku_3262). The genes involved in coenzyme A production from pantothenate are also present in the D. kuznetsovii genome (Desku_1254, 1307, 3145, 3200). Moreover, genes involved in the biosynthesis of pyridoxine (vitamin B6) via the deoxyxylulose 5-phosphate (DXP) independent route were found to be in the genome (Desku_0007, 0008). These genes code for two enzymes that facilitate the conversion of glutamine to the active form of vitamin B6, pyridoxal 5’-phosphate [51].

Menaquinone (vitamin K) and ubiquinone (coenzyme Q10) biosynthesis is important because of the electron transport function in the membranes. The genes that code for the biosynthesis enzymes from polyprenyldiphosphate to menaquinone and ubiquinone are present in the D. kuznetsovii genome (Desku_0124, 0126, 0629, 1551-1554, 1829, 2629 and 3525), except for the genes that code for a 2-polyprenyl-6-methoxyphenol 4-monooxygenase (UbiH) and 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone hydroxylase (UbiF). Additionally, three genes (Desku_1548-1550) could be identified as putative menaquinone biosynthesis genes and are part of a menaquinone biosynthesis gene cluster (Desku_1548-1554). The products of those three genes could be involved in the reactions of the missing UbiH and UbiF encoding genes.

Folate (vitamin B9) biosynthesis is also of great importance for D. kuznetsovii, because it is an essential part of the acetyl-CoA pathway. It is involved in the transfer of one-carbon compounds and can be biosynthesized from chorismate and guanosine triphosphate (GTP) [52-55]. Both pathways use a dihydropteroate synthase to produce dihydropteroate. The genome of D. kuznetsovii contains the genes encoding the enzymes involved in the pathway from chorismate to dihydropteroate (Desku_0219, 2268-2269) and from GTP to dihydropteroate (Desku_0210, 0219-0221 and 1419). The gene encoding a phosphatase (Desku_0210) in the D. kuznetsovii genome is probably involved in the removal of phosphate groups from dihydropterine triphosphate as a substitute for an alkaline phosphatase encoding gene, which is not present in the genome. Additionally, the genome contains a bifunctional protein encoding gene (Desku_404) that is expected to be responsible for the production of dihydrofolate (DHF) and the addition of multiple glutamate moieties to DHF or tetrahydrofolate (THF). However, the D. kuznetsovii genome lacks the DHF reductase encoding gene, which is required to reduce DHF to THF. The DHF reductase encoding gene appears to be absent in many microorganisms [56]. Levin et al. (2004) propose that in Halobacterium salinarum a dihydrofolate synthase and a dihydropteroate synthase domain is able to replace the function of the DHF reductase. Additionally, the authors show that when using a BLAST search, homologs of polypeptides can be found in organisms that lack a DHF reductase [56]. However, BLAST results showed no homologous protein encoding gene in the genome of D. kuznetsovii (data not shown). How in D. kuznetsovii DHF is reduced to THF can currently not be deduced from the genome sequence.

Declarations

Acknowledgements

The work conducted by the U.S. Department of Energy Joint Genome Institute is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231, and was also supported by grants CW-TOP 700.55.343 and ALW 819.02.014 of the Netherlands Science Foundation (NWO) and grant 323009 of the European Research Council.

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