Open Access

Genome sequence of the clover-nodulating Rhizobium leguminosarum bv. trifolii strain TA1

  • Wayne Reeve
  • , Rui Tian
  • , Sofie De Meyer
  • , Vanessa Melino
  • , Jason Terpolilli
  • , Julie Ardley
  • , Ravi Tiwari
  • , John Howieson
  • , Ronald Yates,
  • , Graham O’Hara
  • , Mohamed Ninawi
  • , Hazuki Teshima
  • , David Bruce
  • , Chris Detter
  • , Roxanne Tapia
  • , Cliff Han
  • , Chia-Lin Wei
  • , Marcel Huntemann
  • , James Han
  • , I-Min Chen
  • , Konstantinos Mavromatis
  • , Victor Markowitz
  • , Natalia Ivanova
  • , Galina Ovchinnikova
  • , Ioanna Pagani
  • , Amrita Pati
  • , Lynne Goodwin
  • , Sam Pitluck
  • , Tanja Woyke
  • and Nikos Kyrpides
Corresponding author

DOI: 10.4056/sigs.4488254

Received: 15 December 2013

Accepted: 15 December 2013

Published: 20 December 2013


Rhizobium leguminosarum bv. trifolii strain TA1 is an aerobic, motile, Gram-negative, non-spore-forming rod that is an effective nitrogen fixing microsymbiont on the perennial clovers originating from Europe and the Mediterranean basin. TA1 however is ineffective with many annual and perennial clovers originating from Africa and America. Here we describe the features of R. leguminosarum bv. trifolii strain TA1, together with genome sequence information and annotation. The 8,618,824 bp high-quality-draft genome is arranged in a 6 scaffold of 32 contigs, contains 8,493 protein-coding genes and 83 RNA-only encoding genes, and is one of 20 rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Community Sequencing Program.


root-nodule bacterianitrogen fixationrhizobiaAlphaproteobacteria


Biological fixation of inert atmospheric dinitrogen gas is a process that can only be performed by certain prokaryotes in the domains Archaea and Bacteria. By far the greatest amounts of nitrogen (N) are fixed by specialized soil bacteria (root nodule bacteria or rhizobia) that form proto-cooperative, non-obligatory symbiotic relationships with legumes [1]. Indeed, these symbioses contribute ~40 million tonnes of N annually to support global food production [2].

Species of the legume genus Trifolium (clovers) are amongst the most widely cultivated pasture legumes. Naturally, this genus inhabits three distinct centers of diversity with approximately 28% of species in the Americas, 57% in Eurasia and 15% in Sub-Saharan Africa [3]. A smaller subset of about 30 species, almost all of Eurasian origin, are widely gown as annual and perennial species in pasture systems in Mediterranean and temperate regions [3]. Globally important perennial species of clover include T. repens (white clover), T. pratense (red clover), T. fragiferum (strawberry clover) and T. hybridum (alsike clover). Clovers usually form N2-fixing symbioses with the common soil bacterium Rhizobium leguminosarum bv. trifolii, and different combinations of Trifolium hosts and strains of R. leguminosarum bv. trifolii can vary markedly in symbiotic compatibility [4], resulting in a broad range of symbiotic developmental outcomes ranging from ineffective (non-nitrogen fixing) nodulation to fully effective N2-fixing partnerships [5].

In Australia, Rhizobium leguminosarum bv. trifolii strain TA1 (initially designated BA-Tas) has a long history of use as a commercial inoculant for Trifolium spp. [6]. TA1 was originally isolated from a root nodule on the annual species T. subterraneaum in Bridport, Tasmania in the early 1950’s [6]. This isolate is likely to be a naturalized strain of European origin that arrived by chance in Tasmania in the 1800’s. Although widely used as a microsymbiont of European clovers, it became evident that this soil saprophyte is not acid tolerant [7] and survives poorly when coated onto clover seed with a peat based carrier [8-10]. Nevertheless, TA1 remains the commercial inoculant in Australia for perennial (T repens, T. pratense, T. fragiferum, T. hybridum, T. tumens (talish clover)) and annual (T. alexandrinum (berseem clover), T. glomeratum (cluster clover) and T. dubium (suckling clover)) clovers of European origin [11]. Furthermore, this R. leguminosarum bv. trifolii strain has been adopted by the international community as a model organism to investigate the biology of the Trifolium-Rhizobium symbiosis [12]. Here we present a summary classification and a set of general features for R. leguminosarum bv. trifolii strain TA1 together with the description of the complete genome sequence and its annotation.

Classification and general features

R. leguminosarum bv. trifolii strain TA1 is a motile, Gram-negative, non-spore-forming rod (Figure 1 Left and Center) in the order Rhizobiales of the class Alphaproteobacteria. It is slow growing, forming 1-4 mm diameter colonies within 3-5 days grown on half Lupin Agar (½LA) [13] at 28°C. Colonies on ½LA are white-opaque, slightly domed, moderately mucoid with smooth margins (Figure 1 Right). Minimum Information about the Genome Sequence (MIGS) is provided in Table 1. Figure 2 shows the phylogenetic neighborhood of R. leguminosarum bv. trifolii strain TA1 in a 16S rRNA sequence based tree. This strain clusters closest to R. leguminosarum bv. trifolii T24 and R. leguminosarum bv. phaseoli RRE6 with 99.9% and 99.8% sequence identity, respectively.

Figure 1

Images of Rhizobium leguminosarum bv. trifolii strain TA1 using scanning (Left) and transmission (Center) electron microscopy as well as light microscopy to visualize colony morphology on solid media (Right).

Table 1

Classification and general features of Rhizobium leguminosarum bv. trifolii strain TA1 according to the MIGS recommendations [14].




    Evidence code

    Current classification

    Domain Bacteria

    TAS [15]

    Phylum Proteobacteria

    TAS [16]

    Class Alphaproteobacteria

    TAS [17,18]

    Order Rhizobiales

    TAS [17,19]

    Family Rhizobiaceae

    TAS [20,21]

    Genus Rhizobium

    TAS [20,22-25]

    Species Rhizobium leguminosarum bv. trifolii

    TAS [20,22,25,26]

    Gram stain


    TAS [27]

    Cell shape


    TAS [27]



    TAS [27]



    TAS [27]

    Temperature range


    TAS [27]

    Optimum temperature


    TAS [27]


    Not reported


    Oxygen requirement


    TAS [27]

    Carbon source


    Energy source


    TAS [27]



    Soil, root nodule, on host



    Biotic relationship

    Free living, symbiotic





    TAS [27]

    Biosafety level


    TAS [28]


    Root nodule of Trifolium subterraneum

    TAS [29]


    Geographic location

    Bridport, Tasmania



    Nodule collection date













    Not recorded



    Not recorded

Evidence codes – IDA: Inferred from Direct Assay; TAS: Traceable Author Statement (i.e., a direct report exists in the literature). These evidence codes are from the Gene Ontology project [30].

Figure 2

Phylogenetic tree showing the relationship of Rhizobium leguminosarum bv. trifolii strain TA1 (shown in blue print) with some of the root nodule bacteria in the order Rhizobiales based on aligned sequences of the 16S rRNA gene (1,307 bp internal region). All sites were informative and there were no gap-containing sites. Phylogenetic analyses were performed using MEGA, version 5.05 [31]. The tree was built using the maximum likelihood method with the General Time Reversible model. Bootstrap analysis [32] with 500 replicates was performed to assess the support of the clusters. Type strains are indicated with a superscript T. Strains with a genome sequencing project registered in GOLD [33] are in bold print and the GOLD ID is mentioned after the accession number. Published genomes are designated with an asterisk.


Rhizobium leguminosarum bv. trifolii strain TA1 is currently the commercial inoculant for white (Trifolium repens), red (Trifolium pratense) and strawberry (Trifolium fragiferum) clovers in Australia. TA1 in general is not as effective for nitrogen fixation on annual clovers as other strains, such as WSM1325 [34,35]. However TA1 is of particular interest because it displays a broad host range for nodulation and nitrogen fixation across annual and perennial clovers originating from the European and Mediterranean centre of origin of clovers [1]. TA1 is generally able to nodulate but unable to fix with many annual and and perennial clovers originating from Africa and America [34].

Genome sequencing and annotation information

Genome project history

This organism was selected for sequencing on the basis of its environmental and agricultural relevance to issues in global carbon cycling, alternative energy production, and biogeochemical importance, and is part of the Community Sequencing Program at the U.S. Department of Energy, Joint Genome Institute (JGI) for projects of relevance to agency missions. The genome project is deposited in the Genomes OnLine Database [33] and an improved-high-quality-draft genome sequence in IMG. Sequencing, finishing and annotation were performed by the JGI. A summary of the project information is shown in Table 2.

Table 2

Genome sequencing project information for Rhizobium leguminosarum bv. trifolii strain TA1.





    Finishing quality

    Improved high-quality draft


    Libraries used

    Illumina GAii shotgun and paired end 454 libraries


    Sequencing platforms

    Illumina GAii and 454 GS FLX Titanium technologies


    Sequencing coverage

    7.8× 454 paired end, 764.2× Illumina



    Velvet 1.0.13, Newbler 2.3, phrap 4.24


    Gene calling methods

    Prodigal 1.4, GenePRIMP



    NCBI project ID


    Database: IMG


    Project relevance

    Symbiotic N2 fixation, agriculture

Growth conditions and DNA isolation

Rhizobium leguminosarum bv. trifolii strain TA1 was grown to mid logarithmic phase in TY rich media [36] on a gyratory shaker at 28°C. DNA was isolated from 60 ml of cells using a CTAB (Cetyl trimethyl ammonium bromide) bacterial genomic DNA isolation method [37].

Genome sequencing and assembly

The genome of Rhizobium leguminosarum bv. trifolii strain TA1 was sequenced at the Joint Genome Institute (JGI) using a combination of Illumina [38] and 454 technologies [39]. An Illumina GAii shotgun library which generated 66,421,308 reads totaling 5,048 Mb, and a paired end 454 library with an average insert size of 13 kb which generated 393,147 reads totaling 100.1 Mb of 454 data were generated for this genome. All general aspects of library construction and sequencing performed at the JGI can be found at the JGI user homepage [40]. The initial draft assembly contained 199 contigs in 5 scaffolds. The 454 paired end data was assembled with Newbler, version 2.3. The Newbler consensus sequences were computationally shredded into 2 kb overlapping fake reads (shreds). Illumina sequencing data were assembled with VELVET, version 1.0.13 [41], and the consensus sequence were computationally shredded into 1.5 kb overlapping fake reads (shreds). We integrated the 454 Newbler consensus shreds, the Illumina VELVET consensus shreds and the read pairs in the 454 paired end library using parallel phrap, version SPS - 4.24 (High Performance Software, LLC). The software Consed [42-44] was used in the following finishing process. Illumina data was used to correct potential base errors and increase consensus quality using the software Polisher developed at JGI (Alla Lapidus, unpublished). Possible mis-assemblies were corrected using gapResolution (Cliff Han, unpublished), Dupfinisher (Han, 2006), or sequencing cloned bridging PCR fragments with subcloning. Gaps between contigs were closed by editing in Consed, by PCR and by Bubble PCR (J-F Cheng, unpublished) primer walks. A total of 275 additional reactions were necessary to close gaps and to raise the quality of the finished sequence. The estimated genome size is 7.6 Mb and the final assembly is based on 65.3 Mb of 454 draft data which provides an average of 8.6× coverage of the genome and 4,864.7 Mb of Illumina draft data which provides an average 640.1× coverage of the genome.

Genome annotation

Genes were identified using Prodigal [45] as part of the DOE-JGI Annotation pipeline [46], followed by a round of manual curation using the JGI GenePRIMP pipeline [47]. The predicted CDSs were translated and used to search the National Center for Biotechnology Information (NCBI) non-redundant database, UniProt, TIGRFam, Pfam, PRIAM, KEGG, COG, and InterPro databases. These data sources were combined to assert a product description for each predicted protein. Non-coding genes and miscellaneous features were predicted using tRNAscan-SE [48], RNAMMer [49], Rfam [50], TMHMM [51], and SignalP [52]. Additional gene prediction analyses and functional annotation were performed within the Integrated Microbial Genomes (IMG-ER) platform [37,53].

Genome properties

The genome is 8,618,824 nucleotides with 60.74% GC content (Table 3) and comprised of 32 contigs in 6 scaffolds (Figure 3). From a total of 8,576 genes, 8,493 were protein encoding and 83 RNA only encoding genes. The majority of genes (77.85%) were assigned a putative function whilst the remaining genes were annotated as hypothetical. The distribution of genes into COGs functional categories is presented in Table 4.

Table 3

Genome sequencing project information for Rhizobium leguminosarum bv. trifolii strain SRDI943.



    % of Total

Genome size (bp)



DNA coding region (bp)



DNA G+C content (bp)



Number of scaffolds


Number of contigs


Total genes



RNA genes



rRNA operons*



Protein-coding genes



Genes with function prediction



Genes assigned to COGs



Genes assigned Pfam domains



Genes with signal peptides



Genes with transmembrane helices



CRISPR repeats


*1 copy of 23S rRNA, 2 copies of 16S and 2 copies of 5S rRNA genes

Figure 3

Graphical linear map of the genome of Rhizobium leguminosarum bv. trifolii strain TA1. From outside to the center: Genes on forward strand (color by COG categories), Genes on reverse strand (color by COG categories), RNA genes (tRNAs green, sRNAs red, other RNAs black), GC content, GC skew.

Table 4

Number of protein coding genes of Rhizobium leguminosarum bv. trifolii TA1 associated with the general COG functional categories.




       COG Category




       Translation, ribosomal structure and biogenesis




       RNA processing and modification








       Replication, recombination and repair




       Chromatin structure and dynamics




       Cell cycle control, mitosis and meiosis




       Nuclear structure




       Defense mechanisms




       Signal transduction mechanisms




       Cell wall/membrane biogenesis




       Cell motility








       Extracellular structures




       Intracellular trafficking and secretion




       Posttranslational modification, protein turnover, chaperones




       Energy production conversion




       Carbohydrate transport and metabolism




       Amino acid transport metabolism




       Nucleotide transport and metabolism




       Coenzyme transport and metabolism




       Lipid transport and metabolism




       Inorganic ion transport and metabolism




       Secondary metabolite biosynthesis, transport and catabolism




       General function prediction only




       Function unknown




       Not in COGS



This work was performed under the auspices of the US Department of Energy’s Office of Science, Biological and Environmental Research Program, and by the University of California, Lawrence Berkeley National Laboratory under contract No. DE-AC02-05CH11231, Lawrence Livermore National Laboratory under Contract No. DE-AC52-07NA27344, and Los Alamos National Laboratory under contract No. DE-AC02-06NA25396. We gratefully acknowledge the funding received from the Murdoch University Strategic Research Fund through the Crop and Plant Research Institute (CaPRI) and the Centre for Rhizobium Studies (CRS) at Murdoch University. The authors would like to thank the Australia-China Joint Research Centre for Wheat Improvement (ACCWI) and SuperSeed Technologies (SST) for financially supporting Mohamed Ninawi’s PhD project.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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