Open Access

Genome sequence of Phaeobacter inhibens type strain (T5T), a secondary metabolite producing representative of the marine Roseobacter clade, and emendation of the species description of Phaeobacter inhibens

  • Marco Dogs
  • , Sonja Voget
  • , Hazuki Teshima
  • , Jörn Petersen
  • , Karen Davenport
  • , Hajnalka Dalingault
  • , Amy Chen
  • , Amrita Pati
  • , Natalia Ivanova
  • , Lynne A. Goodwin,
  • , Patrick Chain
  • , John C. Detter,
  • , Sonja Standfest
  • , Manfred Rohde
  • , Sabine Gronow
  • , Nikos C. Kyrpides
  • , Tanja Woyke
  • , Meinhard Simon
  • , Hans-Peter Klenk
  • , Markus Göker
  • and Thorsten Brinkhoff
Corresponding author

DOI: 10.4056/sigs.4448212

Received: 15 December 2013

Accepted: 15 December 2013

Published: 20 December 2013

Abstract

Strain T5T is the type strain of the species Phaeobacter inhibens Martens et al. 2006, a secondary metabolite producing bacterium affiliated to the Roseobacter clade. Strain T5T was isolated from a water sample taken at the German Wadden Sea, southern North Sea. Here we describe the complete genome sequence and annotation of this bacterium with a special focus on the secondary metabolism and compare it with the genomes of the Phaeobacter inhibens strains DSM 17395 and DSM 24588 (2.10), selected because of the close phylogenetic relationship based on the 16S rRNA gene sequences of these three strains. The genome of strain T5T comprises 4,130,897 bp with 3.923 protein-coding genes and shows high similarities in genetic and genomic characteristics compared to P. inhibens DSM 17395 and DSM 24588 (2.10). Besides the chromosome, strain T5T possesses four plasmids, three of which show a high similarity to the plasmids of the strains DSM 17395 and DSM 24588 (2.10). Analysis of the fourth plasmid suggested horizontal gene transfer. Most of the genes on this plasmid are not present in the strains DSM 17395 and DSM 24588 (2.10) including a nitrous oxide reductase, which allows strain T5T a facultative anaerobic lifestyle. The G+C content was calculated from the genome sequence and differs significantly from the previously published value, thus warranting an emendation of the species description.

Keywords:

Anaerobicmotilerod-shapedtropodithietic acidsecondary metabolitesRhodobacteralesRhodobacteraceae

Introduction

Strain T5T was isolated from a water sample taken on 25th of October 1999 above an intertidal mud flat of the German Wadden Sea (53°42’20’’N, 07°43’11’’E) and found to be closely related to the type strain of Roseobacter gallaeciensis [1]. Two years later Martens et al. (2006) reclassified Roseobacter gallaeciensis as Phaeobacter gallaeciensis and described strain T5T as type strain of the species Phaeobacter inhibens. As found for various Phaeobacter strains [2-7], P. inhibens strain T5T (= DSM 16374T = LMG 22475T = CIP 109289T) is able to produce the antibiotic tropodithietic acid (TDA) [8]. Furthermore, strains of P. gallaeciensis and P. inhibens, including strain T5T, are able to produce a brownish pigment, which is the basis of the genus name (phaeos = dark, brown) [1]. The epithet of the species name points to the strong inhibitory activity of P. inhibens against different taxa of marine bacteria and algae [1]. The genus Phaeobacter is known to have a high potential for secondary metabolite production, as indicated by biosynthesis of TDA and N-acyl homoserine lactones (AHLs), as well as presence of genes coding for polyketide synthases (PKS) and nonribosomal peptide synthetases (NRPS) [2,7-10]. Biosynthesis of many different bioactive natural products is mediated by PKSs or NRPSs, including antibiotics, toxins and siderophores. Moreover, production of volatile compounds is widespread over the Roseobacter clade. It displays a particularly high proportion of volatile sulfur-containing compounds and thus seems to play an important role in the sulfur cycle of the ocean [11]. The sulfur-containing TDA, for which the sulfur precursor has not yet been determined, plays an important role in the mutualistic symbioses of P. inhibens and marine algae [12]. p-Coumaric acid causes the organism to switch from a state of mutualistic symbiosis to a pathogenic lifestyle in which toxicity is mediated via the production of the algicidal roseobacticides, which, like p-coumaric, is also a sulfur-containing metabolite [13,14].

Here we present the genome of P. inhibens strain T5T with particular emphasis on the genes involved in secondary metabolism and comparison with the recently published genomes of the P. inhibens strains DSM 17395 and DSM 24588 (2.10) [3]. DSM 17395 and DSM 24588, originally deposited as P. gallaeciensis strains, were recently reclassified as P. inhibens [15].

Classification and features

16S rRNA gene analysis

Figure 1 shows the phylogenetic neighborhood of P. inhibens DSM 16374T in a tree based on 16S rRNA genes. The sequences of the three identical 16S rRNA gene copies differ by one nucleotide from the previously published 16S rRNA sequence (NCBI Accession No. AY177712).

Figure 1

Phylogenetic tree highlighting the position of P. inhibens relative to the type strains of the other species within the genus Phaeobacter and the neighboring genera Leisingera and Ruegeria [1,20-33]. The tree was inferred from 1,385 aligned characters [34,35] of the 16S rRNA gene sequence under the maximum likelihood (ML) criterion [36]. Rooting was done initially using the midpoint method [37] and then checked for its agreement with the current classification (Table 1). The branches are scaled in terms of the expected number of substitutions per site. Numbers adjacent to the branches are support values from 1,000 ML bootstrap replicates [38] (left) and from 1,000 maximum-parsimony bootstrap replicates [39] (right) if larger than 60%. Lineages with type strain genome sequencing projects registered in GOLD [40] are labeled with one asterisk, those also listed as 'Complete and Published' with two asterisks [21]. The genomes of six more Leisingera and Phaeobacter species are published in the current issue of Standard in Genomic Science [41-46]. The 16S rRNA sequences of P. inhibens strain DSM 24588 and P. inhibens strain DSM 17395 are virtually identical to those of P. inhibens DSM 16374T (data not shown).

Table 1

Classification and general features of P. inhibens T5T according to the MIGS recommendations [48].

MIGS ID

    Property

    Term

   Evidence code

    Current classification

    Domain Bacteria

   TAS [49]

    Phylum Proteobacteria

   TAS [50]

    Class Alphaproteobacteria

   TAS [51,52]

    Order Rhodobacterales

   TAS [52]

    Family Rhodobacteraceae

   TAS [52,53]

    Genus Phaeobacter

   TAS [1]

    Species Phaeobacter inhibens

   TAS [1]

    Type strain T5

   TAS [1,8]

    Gram stain

    negative

   TAS [1]

    Cell shape

    rod shaped

   TAS [1]

    Motility

    motile

   TAS [1]

    Sporulation

    none

   TAS [1]

MIGS-6.1

    Temperature range

    mesophile

   TAS [1]

MIGS-6.1

    Optimum temperature

    27-29°C

   TAS [1]

MIGS-6.3

    Salinity

    0.01- <1.5M NaCl

   TAS [1]

MIGS-22

    Oxygen requirement

    facultative anaerobic

   IDA

    Carbon source

    oligosaccharides, sugar alcohols, organic acids, amino acids

   TAS [1]

    Energy metabolism

    heterotrophic

   TAS [1]

MIGS-6

    Habitat

    marine

   TAS [1]

MIGS-15

    Biotic relationship

    unknown

   NAS

MIGS-14

    Pathogenicity

    none

   TAS [1]

    Biosafety level

    1

   TAS [54]

MIGS-23

    Isolation

    water sample above an intertidal mud flat

   TAS [1,8]

MIGS-4

    Geographic location

    German Wadden Sea

   TAS [1,8]

MIGS-5

    Sample collection time

    October 25, 1999

   TAS [1,8]

MIGS-4.1

    Latitude

    53°42´20´´N

   TAS [1,8]

MIGS-4.2

    Longitude

    07°43´11´´E

   TAS [1,8]

MIGS-4.3

    Depth

    Above sea ground

   TAS [1,8]

MIGS-4.4

    Altitude

    unknown

Evidence codes – IDA: Inferred from direct assay; TAS: Traceable Author Statement (i.e., a direct report exists in the literature); NAS: Non-traceable Author Statement (i.e., not directly observed for the living, isolated sample, but based on a generally accepted property for the species, or anecdotal evidence). Evidence codes are from the Gene Ontology project [55].

A representative genomic 16S rRNA gene sequence of P. inhibens DSM 16374T was compared using NCBI BLAST [16,17] under default settings (e.g., considering only the high-scoring segment pairs (HSPs) from the best 250 hits) with the most recent release of the Greengenes database [18] and the relative frequencies of taxa and keywords (reduced to their stem [19]) were determined, weighted by BLAST scores. The most frequently occurring genera were Ruegeria (32.5%), Phaeobacter (28.8%), Silicibacter (13.6%), Roseobacter (13.3%) and Nautella (3.5%) (141 hits in total). Regarding the single hit to sequences from the species, the average identity within HSPs was 99.8%, whereas the average coverage by HSPs was 99.3%. Regarding the nine hits to sequences from other species of the genus, the average identity within HSPs was 99.0%, whereas the average coverage by HSPs was 99.2%. Among all other species, the one yielding the highest score was P. gallaeciensis (NZ_ABIF01000004), which corresponded to an identity of 100.0% and an HSP coverage of 100.0%. (Note that the Greengenes database uses the INSDC (= EMBL/NCBI/DDBJ) annotation, which is not an authoritative source for nomenclature or classification). The highest-scoring environmental sequence was AJ296158 (Greengenes short name 'Spain:Galicia isolate str. PP-154'), which showed an identity of 99.8% and an HSP coverage of 100.0%. The most frequently occurring keywords within the labels of all environmental samples which yielded hits were 'microbi' (3.1%), 'marine' (2.6%), 'coral' (2.3%), 'biofilm' (2.1%) and 'membrane, structure, swro' (1.8%) (100 hits in total). Environmental samples which yielded hits of a higher score than the highest scoring species were not found.

Morphology and physiology

Cells of T5T are ovoid rods, 1.4-1.9 x 0.6-0.8 µm (Figure 2). Furthermore, T5T cells show the typical multicellular star-shaped structure described previously for P. gallaeciensis and other Roseobacter-clade organisms [2,4,47] (Figure 2). Cells of T5T are motile by means of a polar flagellum. T5T is a Gram-negative, marine, facultatively anaerobic, mesophilic bacterium with an optimal growth temperature between 27 and 29 °C and an optimal salinity between 0.51 and 0.68 M. The pH range for growth is 6.0 – 9.5, with an optimum at 7.5. On marine agar T5T forms smooth and convex colonies with regular edges and brownish pigmentation on ferric citrate containing media. T5T utilizes pentoses, hexoses, disaccharides and most amino acids as carbon and energy sources. No vitamin requirements were observed [1].

Figure 2

Scanning electron microscope pictures of P. inhibens strain DSM 16374T showing (a) the typical cell ovoid shape of strain T5T and (b) the multicellular, star-shaped structure as described previously for Phaeobacter and further Roseobacter-clade organisms.

Chemotaxonomy

There are no significant differences between the fatty-acid profile of strain T5T and other representatives of the Roseobacter clade [1]. Strain T5T has the highest profile similarity to P. gallaeciensis CIP 105210T [1]. The principal cellular fatty acids of strain T5T are the following saturated branched-chain fatty acids: C18:1ω7c (73.77%), 11-methyl C18:1ω7c (7.45%), C16:0 (3.83%), C18:0 (3.14%), 2-OH C16:0 (3.10%), C14:1 (2.19%), 3-OH C10:0 (1.71%), 3-OH C12:0 (1.59%), 3-OH C14:1/ 3 oxo-C14:0 (0.87%), C18.1ω9c (0.76%) and an unambiguously identified fatty acid (1.59%) [1]. The major polar lipids of strain T5T comprise phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an aminolipid and two unidentified lipids [1].

Genome sequencing and annotation

Genome project history

This organism was selected for sequencing on the basis of the DOE Joint Genome Institute Community Sequencing Program (CSP) 2010, CSP 441 “Whole genome type strain sequences of the genera Phaeobacter and Leisingera – a monophyletic group of physiological highly diverse organisms”. The genome project is deposited in the Genomes On Line Database [40] and the complete genome sequence is deposited in GenBank and the Integrated Microbial Genomes database (IMG) [56]. Sequencing, finishing and annotation were performed by the DOE Joint Genome Institute (JGI) using state of the art sequencing technology [57]. A summary of the project information is shown in Table 2.

Table 2

Genome sequencing project information

MIGS ID

   Property

   Term

MIGS-31

   Finishing quality

   Permanent draft

MIGS-28

   Libraries used

   Two Illumina paired-end libraries (225 bp and 9kb insert size)

MIGS-29

   Sequencing platforms

   Illumina GAii, PacBio

MIGS-31.2

   Sequencing coverage

   1,111 × Illumina

MIGS-30

   Assemblers

   Allpaths, Velvet 1.1.05, phrap version SPS - 4.24

MIGS-32

   Gene calling method

   Prodigal 1.4, GenePRIMP

   INSDC ID

   AXBB00000000

   GenBank Date of Release

   September 30, 2013

   GOLD ID

   Gi10860

   NCBI project ID

   88111

   Database: IMG-GEBA

   2516653078

MIGS-13

   Source material identifier

   DSM 16374

   Project relevance

   Tree of Life, carbon cycle, sulfur cycle, environmental

Growth conditions and DNA isolation

A culture of DSM 16374T was grown aerobically in DSMZ medium 514 [58] at 25°C. Genomic DNA was isolated using the Jetflex Genomic DNA Purification Kit (GENOMED 600100) following the standard protocol provided by the manufacturer but modified by an incubation time of 40 min, the incubation on ice over night on a shaker, the use of additional 10 µl proteinase K, and the addition of 100 µl protein precipitation buffer. DNA is available from DSMZ through the DNA Bank Network [59].

Genome sequencing and assembly

For this genome, we constructed and sequenced an Illumina short-insert paired-end library with an average insert size of 225 bp, and an Illumina long-insert paired-end library with an average insert size of 9602 bp, which generated 18,471,132 reads and 11,906,846 reads, respectively, totaling 4,557 Mbp of Illumina data. All general aspects of library construction and sequencing performed can be found at the JGI website [60]. The initial draft assembly contained 13 contigs in 10 scaffold. The initial draft data was assembled with Allpaths [61] and the consensus was computationally shredded into 10 kbp overlapping fake reads (shreds). The Illumina draft data was also assembled with Velvet [62], and the consensus sequences were computationally shredded into 1.5 kbp overlapping fake reads (shreds). The Illumina draft data was assembled again with Velvet using the shreds from the first Velvet assembly to guide the next assembly. The consensus from the second Velvet assembly was shredded into 1.5 kbp overlapping fake reads. The fake reads from the Allpaths assembly and both Velvet assemblies and a subset of the Illumina CLIP paired-end reads were assembled using parallel phrap (High Performance Software, LLC) [63]. Possible mis-assemblies were corrected with manual editing in Consed [63]. Gap closure was accomplished using repeat resolution software (Wei Gu, unpublished), and sequencing of bridging PCR fragments with PacBio technologies. A total of 10 PCR PacBio consensus sequences were completed to close gaps and to raise the quality of the final sequence. The final assembly is based on 4,557 Mbp of Illumina draft data, which provides an average 1,111 × coverage of the genome.

Genome annotation

Genes were identified using Prodigal [64] as part of the DOE-JGI genome annotation pipeline [65], followed by a round of manual curation using the JGI GenePRIMP pipeline [66]. The predicted CDSs were translated and used to search the National Center for Biotechnology Information (NCBI) nonredundant database, UniProt, TIGR-Fam, Pfam, PRIAM, KEGG, COG, and InterPro databases. Additional gene prediction analysis and functional annotation were performed within the Integrated Microbial Genomes - Expert Review (IMG-ER) platform [56].

Genome properties

The genome statistics are provided in Table 3 and Figure 3. The genome consists of six scaffolds with a total length of 4,130,897 bp and a G+C content of 60.0%. The scaffolds correspond to a chromosome 3,669,861 bp in length and four extrachromosomal elements as identified by their replication systems (see below). Of the 3,986 genes predicted, 3,923 were protein-coding genes, and 63 RNAs; 39 pseudogenes were also identified. The majority of the protein-coding genes (81.0%) were assigned a putative function while the remaining ones were annotated as hypothetical proteins. The distribution of genes into COGs functional categories is presented in Table 4.

Table 3

Genome Statistics

Attribute

   Value

   % of total

Genome size (bp)

   4.130.897

   100.00%

DNA coding region (bp)

   3.683.922

   89.18%

DNA G+C content (bp)

   2.479.086

   60.02%

Number of scaffolds

   6*

Extrachromosomal elements

   4

Total genes

   3.986

   100.00%

RNA genes

   63

   1.58%

rRNA genes

   5*

   0.13%

rRNA operons

   1*

Protein-coding genes

   3.923

   98.42%

Pseudo genes

   39

   0.98%

Genes with function prediction

   3.228

   80.98%

Genes in paralog clusters

   1.234

   30.96%

Genes assigned to COGs

   3.178

   79.73%

Genes assigned Pfam domains

   3.395

   85.17%

Genes with signal peptides

   306

   7.68%

Genes with transmembrane helices

   841

   21.10%

CRISPR repeats

   0

* An updated version of the genome assembly (unpublished data) reveals the presence of four rRNA operons. In this version, two of the rRNA operons are incomplete, with the 16S rRNA gene and the 5S rRNA gene hidden in the two remaining sequencing gaps. The genomes of P. inhibens strain DSM 24588 (2.10) and P. inhibens strain DSM 17395 (see Figure 3) also contain four rRNA operons.

Figure 3

Graphical representation of the genome of P. inhibens T5T. From outside to the center: (1) sequence of P. inhibens T5T, (2) results of a blastn comparison from P. inhibens DSM 24588 (2.10) against P. inhibens T5T, (3) results of a blastn comparison of P. inhibens DSM 17395 against P. inhibens T5T, (4) G+C content. Comparisons and visualization are done with BRIG [67].

Table 4

Number of genes associated with the general COG functional categories

Code

   Value

   %age

     Description

J

   170

   4.87

     Translation, ribosomal structure and biogenesis

A

   1

   0.03

     RNA processing and modification

K

   274

   7.85

     Transcription

L

   137

   3.92

     Replication, recombination and repair

B

   3

   0.09

     Chromatin structure and dynamics

D

   29

   0.83

     Cell cycle control, cell division, chromosome partitioning

Y

   n. a.

   n. a.

     Nuclear structure

V

   45

   1.29

     Defense mechanisms

T

   156

   4.47

     Signal transduction mechanisms

M

   199

   5.70

     Cell wall/membrane biogenesis

N

   52

   1.49

     Cell motility

Z

   n. a.

   n. a.

     Cytoskeleton

W

   n. a.

   n. a.

     Extracellular structures

U

   60

   1.72

     Intracellular trafficking and secretion, and vesicular transport

O

   127

   3.64

     Posttranslational modification, protein turnover, chaperones

C

   204

   5.84

     Energy production and conversion

G

   197

   5.64

     Carbohydrate transport and metabolism

E

   425

   12.17

     Amino acid transport and metabolism

F

   79

   2.26

     Nucleotide transport and metabolism

H

   152

   4.35

     Coenzyme transport and metabolism

I

   146

   4.18

     Lipid transport and metabolism

P

   174

   4.98

     Inorganic ion transport and metabolism

Q

   110

   3.15

     Secondary metabolites biosynthesis, transport and catabolism

R

   446

   12.77

     General function prediction only

S

   306

   8.76

     Function unknown

-

   808

   20.27

     Not in COGs

Insights into the genome

Genome sequencing of P. inhibens DSM 16374T revealed the presence of four extrachromosomal elements with sizes of 227 kb, 88 kb, 78 kb, and 69 kb (Figure 3; Table 5) and DnaA-like I, RepABC-8, RepB-I and RepA-I as replication systems, respectively [68]. The different replicases that mediate the initiation of replication are designated according to the established plasmid classification scheme [69]. With the exception of the 88 kb replicon, these extrachromosomal elements are highly syntenic to specific replicons in the genomes of P. inhibens strains DSM 17395 and DSM 24588 (Figure 3).

Table 5

General genomic features of the chromosome and extrachromosomal replicons of Phaeobacter inhibens strain DSM 16374T

Replicon

   Scaffold

    Length (bp)

    GC (%)

    Topology

   No. Genes#

cInhi_A3361

   1

    3,361,358

    60

    linear*

   3,282

cInhi_B309

   2

    308,503

    61

    linear*

   288

pInhi_A227

   3

    226,687

    59

    linear*

   208

pInhi_B88

   4

    87,579

    58

    linear*

   93

pInhi_C78

   5

    78,203

    63

    linear*

   62

pInhi_D69

   6

    68,567

    63

    linear*

   53

#deduced from automatic annotation

*circularity not experimentally validated.

The locus tags of all replicases, plasmid stability modules and the large virB4 gene of a type IV secretion system are presented in Table 6. The plasmids pInhi_A227 and pInhi_B88 contain postsegregational killing systems (PSK) consisting of a typical operon with two small genes encoding a stable toxin and an unstable antitoxin [70]. Moreover, plasmid pInhi_B88 also contains a complete virB gene cluster of type IV secretion system, required for the formation of a transmembrane channel. However, the absence of the relaxase VirD2, which is necessary for the strand-specific DNA nicking at the origin of transfer (oriT), and the coupling protein VirD4 indicates that this plasmid is non-conjugative [71,72]. The RepA-I type replicon pInhi_D69 contains a complete rhamnose operon [73] and is dominated by genes required for polysaccharide biosynthesis.

Table 6

Integrated Microbial Genome (IMG) locus tags of P. inhibens DSM 16374T genes for the initiation of replication, toxin/antitoxin modules and two representatives of type IV secretion systems (T4SS) that are required for conjugation.

Replicon

     Replication Initiation

     Plasmid Stability

     Type IV Secretion

     Replicon

     Replication Initiation

     Plasmid Stability

     Replicase

     Locus Tag

     Toxin

     Antitoxin

     VirB4

     VirD4

cInhi_A3361

     DnaA

     Inhi_2434

     -

     -

     -

     -

cInhi_B309

     -

     -

     -

     -

     -

     -

pInhi_A227

     DnaA-like I

     Inhi_3576

     Inhi _3735

     Inhi _3734

     -

     -

pInhi_B88

     RepC-8

     Inhi_3797

     Inhi _3865

     Inhi _3866

     Inhi _3845

     -

pInhi_C78

     RepB-I

     Inhi_3883

     -

     -

     -

     -

pInhi_D69

     RepA-I

     Inhi 3972

     -

     -

     -

     -

As already indicated by the strong inhibitory activity of P. inhibens T5T [8] all 26 described genes involved in the production of TDA are present in the genome of this strain. As found for the P. inhibens strains DSM 17395 and DSM 24588, the key genes for TDA production tdaABCDEF (Inhi_3684 - _3688, Inhi_3701), paaZ2 (Inhi_3702) and a gene coding for a putative Na-dependent transporter (Inhi_3697) [3,74] are located on the 227 kb plasmid of T5T (Figure 3). The remaining 19 genes, containing genes of the phenylacetyl-CoA and assimilatory sulfate reduction pathways, are scattered over the chromosome as in the strains DSM 17395 and DSM 24588 [3]. Beside the tdaA gene, present on the 227 kb plasmid, we also found other genes involved in the regulation of TDA synthesis located on the chromosome, what is in agreement with Thole et al. (2012) and Berger et al. (2012) [3,75]. This includes the genes encoding transcriptional activator proteins (Inhi_2121; _2059; _0396) comparable with pgaR, iorR a transcriptional regulator (PGA1_c20730), a putative serine-protein kinase (Inhi_2265) and a putative signal peptide peptidase (Inhi_2227).

Two complete prophages and an additional cluster coding for the production of gene transfer agents (GTA) were found in the genome of strain T5T. The GTA gene cluster is equal in length and comprises the same genes (Inhi_0654 – Inhi_0670) as the GTA clusters of the strains DSM 17395 and DSM 24588. The two prophages of strain T5T consist of 52 ORFs (prophage 1; ~37kb) and 63 ORFs (prophage 2; ~48kb), respectively. Strain DSM 17395 possesses two prophages, but for DSM 24588 no prophages were detected [3]. Prophage 1 of strain T5T is similar to prophage 1 of strain DSM 17395, with the exception that a few ORFs are different (PGA1_c18280 - _c18310, PGA1_c18480 - _c18530 and PGA1_c18570 - _c18680; Inhi_1777, Inhi_1785 - _1788, Inhi_1803 - _1812 and Inhi_1816 – 1829). Prophage 2 of strain T5T is a Mu-like bacteriophage, not present in strain DSM 17395.

It was previously shown that strain T5T produces two different AHLs, i.e. C18-en-HSL and N-3-hydroxydecanoyl-homoserine lactone (3OHC10-HSL) [76]. In P. inhibens strain DSM 17395 TDA and pigment production are regulated via a pgaR-pgaI QS system [47]. The AHL synthase encoding gene pgaI in DSM 17395 is responsible for the production of 3OHC10-HSL. In the genome of strain T5T we found a homologous system probably coding for the 3OHC10-HSL producing AHL synthase (Inhi_2120, homolog to pgaI) and the respective regulator (Inhi_2121, homologous to pgaR) (Figure 3, QS system I). Thus TDA production in strain T5T might also be regulated by a QS system. In addition, two further QS systems (QS system II and III; Figure 3) were found on the chromosome of T5T. System II is formed by the genes Inhi_0506 and _0507 and is located in the prophage region 2. Orthologs for these QS system genes are also present in P. inhibens strain DSM 24588 (PGA2_c18960 and PGA2_c18970) but absent in strain DSM 17395. QS system III consists of the genes Inhi_1819 and _1820 and is unique for strain T5T compared to P. inhibens DSM 17395 and DSM 24588. It is also located in the potential prophage 1 region (Fig. 3). A homologous system was found in the genome of Phaeobacter caeruleus DSM 24564T and the neighboring genes show a high synteny. The location in the prophage region and the high synteny to the system of P. caeruleus suggest a possible gene transfer of this QS system via a bacteriophage. The functions of QS system II and III are currently unknown, but it is likely that the compound C18-en-HSL is produced by one of those systems.

Two functions were suggested that can possibly be used as unique chemotaxonomic markers for the species P. inhibens within the Roseobacter clade [3]. The genes coding for the first of these functions are located on the chromosome and are involved in cell wall development and surface attachment [dltA encoding a D-alanine-poly(phosphoribitol) ligase involved in biosynthesis of D-alanyl-lipoteichoic acid]. The second unique function is the biosynthesis and transport of iron-chelating siderophores, and the encoding genes are located on the plasmid pPGA1_78 and pPGA2_95, respectively. These two clusters are also present in the genome of strain T5T. The siderophore gene cluster (Inhi_3924 – Inhi_3928) is located on the 78 kb plasmid (Fig. 3) and the dltA gene cluster (Inhi_1065 - Inhi_1086) is located on the chromosome (Fig. 3). Screenings in the newly available Roseobacter genomes showed that Leisingera methylohalidivorans DSM 14336 [42] and Leisingera aquimarina DSM 24565 [41] also harbor the genes for siderophore synthesis. The uniqueness of the dltA gene cluster within the species P. inhibens, however, remains and can be used as chemotaxonomic marker.

The existence of genes coding for a polyketide synthase (Inhi_1972) and three non-ribosomal peptide synthetases (Inhi_1072, _1974 and _3983) confirm the results of Martens et al. (2007) [7]. These genes are present in the genomes of strains DSM 17395 and DSM 24588, too (PGA1_c04930 and PGA1_c05350, _c13760, _c28490; PGA2_c05370 and PGA2_c04910, _c13660, _71p110). The genes Inhi_3983 of P. inhibens strain T5T and PGA2_71p110 of P. inhibens strain DSM 24588 are located on the 69 kb plasmid (Fig. 3) and 71 kb plasmid, respectively. In contrast, the homologous gene (PGA1_c28490) of P. inhibens strain DSM 17395 is located on the chromosome.

For the P. inhibens strains DSM 17395 and DSM 24588 a surface-attached lifestyle was inferred from the genome analysis [3]. Even though strain T5T was isolated from a water sample, it exhibits the same genes associated with the biosynthesis and transport of polysaccharides as strains DSM 17395 and DSM 24588. This includes genes described as unique for the strains DSM 17395 and DSM 24588, i.e. a gene coding for a glycosyltransferase-like protein (Inhi_3961) and two ORFs (Inhi_3954 and Inhi_3955) related to a type I secretion system and used for the transport of exopolysaccharides. Production of extracellular polysaccharides is a major factor contributing to surface attachment [77,78]. Thus it appears likely that T5T is also well-adapted to a surface attached lifestyle.

P. inhibens was described as a strictly aerobic bacterium [1]. However, we found genes involved in the dissimilatory nitrate reduction pathway to nitrogen, including the gene coding for a copper containing nitrite reductase (Inhi_3645) and a nitric oxide reductase cluster (Inhi_3648 - Inhi_3654), both located on the replicon pInhi_A227. These genes are also present and located on the largest plasmids of P. inhibens DSM 17395 (PGA1_262p) and P. inhibens DSM 24588 (PGA2_239p) (Figure 3). In addition, P. inhibens strain T5T possesses a gene cluster coding for a nitrous oxide reductase (Inhi_3786 – Inhi_3792) located on the replicon pInhi_B88, which is absent in the strains DSM 17395 and DSM 24588 (Figure 3). Neither strain T5T nor DSM 17395 and DSM 24588 have genes coding for a nitrate reductase. The findings suggest that P. inhibens T5T has a complete dissimilatory nitrite reduction pathway, but is not able to reduce nitrate, as previously described by Martens et al. (2006) [1]. To confirm the results we tested strain T5T for its capability to grow anaerobically with nitrite. Anaerobic marine basal medium was prepared according to Cypionka and Pfennig (1986) [79] and supplemented with nitrite and glucose, both in a final concentration of 5 mM. After two weeks a decrease of nitrite was determined by photometric analysis at 545 nm by using the Griess reaction [80] and an increase of the turbidity was detected (results not shown). Thus it became clear that P. inhibens T5T is able to grow anaerobically with nitrite, suggesting an emended description of this organism as a facultatively anaerobic bacterium.

Phylogenetic analysis shows that P. inhibens and P. gallaeciensis form a cluster together with Phaeobacter arcticus (Figure 1). The cluster is set apart from the cluster comprising Leisingera aquimarina, Leisnigera nanhaiensis, Leisingera methylohalidivorans, Phaeobacter caeruleus and Phaeobacter daeponensis, but the backbone of the 16S rRNA gene tree shown in Figure 1 is rather unresolved. Using the online analysis tool “Genome-to-Genome Distance Calculator 2.0 (GGDC) [81,82], we performed a preliminary phylogenetic analysis of the draft genomes of the type strains of the genera Leisingera and Phaeobacter and the finished genomes of P. inhibens strains DSM 17395 and DSM 24588. Table 7 shows the results of the in silico calculated DNA-DNA hybridization (DDH) similarities of P. inhibens to other Phaeobacter and Leisingera species. In the following analysis, we will refer only to the results of formula 2, as this formula is robust against the use of draft genomes such as AOQA01000000 (CIP 105210T) [83]. The use of GGDC revealed a high similarity of T5T (78%) to the strains P. inhibens DSM 17395 and DSM 24588, but a low similarity to P. gallaeciensis strain CIP 105210T (36%). DSM 17395 and CIP 105210T were previously supposed to be type-strain deposits for P. gallaeciensis [33] and we cross-compared them using GGDC. Formula 2 yielded a similarity of only 38.30% ± 2.50 between these two strains, thus indicating not only that they are not the same strain, but also do not even belong to the same species. The results are in agreement with the study of Buddruhs et al. (2013) [15] showing that strain DSM 17395 is the false deposit and belongs together with DSM 24588 to P. inhibens, whereas CIP 105210T is the correct type-strain deposit for P. gallaeciensis.

Table 7

Digital DDH similarities between P. inhibens T5T and the other Phaeobacter and Leisingera species (including the genome-sequenced type strains and P. inhibens strains DSM 17395 and DSM 24588 [2,10]) calculated in silico with the GGDC server version 2.0 [83].

Reference strain (type strain unless indicated)

   formula 1

   formula 2

   formula 3

P. arcticus DSM 23566T (AXBF00000000)

   17.60±3.30

   21.70±2.44

   17.80±2.95

P. caeruleus DSM 24564T (AXBI00000000)

   19.00±3.36

   20.60±2.46

   19.10±2.99

P. daeponensis DSM 23529T (AXBD00000000)

   20.40±3.41

   22.60±2.46

   20.20±3.03

P. gallaeciensis CIP 105210T (AOQA01000000)

   78.40±3.76

   36.20±2.57

   68.50±3.52

P. inhibens DSM 17395 (CP002976, CP002977, CP002978, CP002979)

   90.60±2.78

   78.50±2.98

   90.90±2.49

P. inhibens DSM 24588 (2.10) (CP002972, CP002973, CP002974, CP002975)

   94.50±2.03

   78.40±2.98

   94.20±1.92

L. aquimarina DSM 24565T (AXBE00000000)

   18.50±3.34

   22.50±2.45

   18.70±2.98

L. methylohalidivorans DSM 14336T (CP006773, CP006774, CP006775)

   19.40±3.38

   22.50±2.45

   19.40±3.01

L. nanhaiensis DSM 24252T (AXBG00000000)

   14.00±3.08

   21.00±2.42

   14.70±2.78

The standard deviations indicate the inherent uncertainty in estimating DDH values from intergenomic distances based on models derived from empirical test data sets (which are always limited in size); see [83] for details. The distance formulas are explained in [82]; formula 2 is recommended, particularly for draft genomes (such as AOQA01000000). The numbers in parentheses are GenBank accession numbers identifying the underlying genome sequences.

The differences in the G+C content (55.7%) published earlier [1] and the value calculated directly from the genome (Table 3) warrants an update of the taxonomic description on P. inhibens [84]. Moreover, genomic and experimental evidence indicates that P. inhibens is not strictly aerobic but facultatively anaerobic.

Conclusion

Emended description of the species Phaeobacter inhibens Martens et al. 2006

The description of the species Phaeobacter inhibens is the one given by Martens et al. 2006 [1], with the following modification. The G+C content, rounded to zero decimal places, is 60%. Phaeobacter inhibens is a facultative anaerobic bacterium by using nitrite reduction.

Declarations

Acknowledgements

The authors gratefully acknowledge the assistance of Iljana Schröder for growing P. inhibens cultures and Evelyne-Marie Brambilla for DNA extraction and quality control (both at the DSMZ). The work conducted by the U.S. Department of Energy Joint Genome Institute was supported by the Office of Science of the U.S. Department of Energy under contract No. DE-AC02-05CH11231; the work conducted by members of the Roseobacter consortium was supported by the German Research Foundation (DFG) Transregio-SFB 51. We also thank the European Commission which supported phenotyping via the Microme project 222886 within the Framework 7 program.


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